Effects and mechanisms of macrophage function by selenizing Chinese angelica polysaccharide
10.16303/j.cnki.1005-4545.2024.09.20
- VernacularTitle:硒化当归多糖对巨噬细胞功能的影响及机制
- Author:
Jie QIAO
1
;
Sige HAINI
;
Ruifeng GAO
;
Ying YANG
;
Zhenzhen GAO
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
- Keywords:
selenizing Chinese angelica polysaccharide;
macrophage;
phagocytic activity;
cytokine
- From:
Chinese Journal of Veterinary Science
2024;44(9):1999-2009
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this experiment was to investigate the effects of Chinese angelica polysaccha-ride(CAP)on peritoneal macrophage function before and after selenization.In the experiment,CAP and selenized Chinese angelica polysaccharide(sCAP)were applied to mouse peritoneal mac-rophages,and the phagocytic activity of macrophages as well as the expression of the cellular phe-notypes F4/80,MHC-Ⅱ,CD80 and CD86 were detected by flow cytometry,and the changes of IL-6,IL-10,NO,MIP-1α,and TNF-α content in the supernatant of macrophage were detected by ELISA.The results showed that the phagocytic activity of peritoneal macrophages was significantly higher in the sCAP mass concentration of 1.96 mg/L than in the other groups(P＜0.05);and the expression of F4/80,MHC-Ⅱ,CD80 and CD86 were significantly higher in the sCAP mass concen-tration of 1.96 mg/L than in the CAP group and cell control group(P＜0.05);the contents of IL-6,IL-10,NO,and MIP-1α in the supernatant of macrophages were significantly higher than those in other groups when the mass concentration of sCAP ranged from 7.81 to 1.96 mg/L(P＜0.05).The results showed that sCAP had an enhancing effect on the immunomodulatory function of ab-dominal macrophages and was optimal at a mass concentration of 1.96 mg/L.
