Construction and cross-protection of marker-free infC mutant of bovine Pasteu-rella multocida type A
10.16303/j.cnki.1005-4545.2024.09.12
- VernacularTitle:牛源A型多杀性巴氏杆菌infC基因缺失株的构建及其交叉免疫保护性
- Author:
Xuxia YANG
1
;
Pei HU
;
Jiaqi HUANG
;
Lixu GAO
;
Ronghua LYU
;
Yangyang QIU
;
Fang HE
;
Yuanyi PENG
;
Nengzhang LI
Author Information
1. 西南大学动物医学院,重庆北碚 400715
- Keywords:
Pasteurella multocida;
infC gene;
mutant;
virulence;
cross-protection
- From:
Chinese Journal of Veterinary Science
2024;44(9):1930-1939
- CountryChina
- Language:Chinese
-
Abstract:
infC gene encodes the translation initiation factor IF3 in bovine Pasteuella multocida,but it whether or not regulation to the virulence and cross-protection in P.multocida is still not well understood.In this study,the infC gene mutant(△infC)derived from bovine P.multocida type A strain CQ2 was constructed using by homologous recombination method.Compared with wild strain,the △infC showed significant increasing in biofilm formation,but the capsule produc-tion,virulence and bacterial loading in organs were significant decreased,and the IL-1β secretion of mouse peritoneal macrophage increased.Along with the infC gene deletion,the expression of genes related to capsule synthesis and LPS synthesis and transport were significantly down-regulated,while that of genes related to biofilm synthesis and outer membrane protein were significantly up-regulated.The inactivated vaccines of wild type and mutant were prepared and mice were immu-nized twice then challenged with wild type strains,respectively.The immuno-protection rate of△in fC inactivated vaccine against bovine P.multocida type A,B and F were 100.0％,83.3％and 0.0％,respectively,and the immuno-protection rate that against rabbit type P.multocida was 33.3％.The results indicated that infC gene could affect the virulence of P.multocida by regula-ting the production of capsule and the expressions of virulence related factors,and the deletion of infC gene conferred a certain cross-protection property of strains.This study provided a certain foundation for the development of P.multocida vaccine.
