Construction and biological characterization of lmo2363 gene deletion strain of Listeria monocytogenes
10.16303/j.cnki.1005-4545.2024.09.11
- VernacularTitle:单增李斯特菌lmo2363基因缺失株的构建及其生物学特性
- Author:
Ji ZHI
1
;
Qing CAO
;
Xuehui ZHAO
;
Haohao ZHANG
;
Ziqiu FAN
;
Yonghui MA
;
Jing DENG
;
Zengwen HE
;
Jinrui MA
;
Kunzhong ZHANG
;
Qian CHONG
;
Caixia WANG
;
Huiwen XUE
;
Huitian GOU
Author Information
1. 甘肃农业大学动物医学院,甘肃兰州,730070
- Keywords:
Listeria monocytogenes;
lmo2363 gene;
biofilm;
stress resistance
- From:
Chinese Journal of Veterinary Science
2024;44(9):1923-1929,1956
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the function of lmo2363 gene in stress resistance of Liste-ria monocytogenes strain LM83-1.In this study,the lmo2363 gene deletion strain and complement-ation strain of Listeria monocytogenes were constructed using overlapping extended PCR and ho-mologous recombination techniques,and the growth ability,stress survival rate and biofilm forma-tion ability of wild,deletion strain and complementation strain were compared under different stress environments.lmo2363 gene deletion strain and complementation strain of Listeria monocy-togenes were successfully constructed in this experiment.The growth curves showed that the growth capacity of the deletion strain was weaker than the wild strain LM83-1 under 4 ℃,7％NaCl,10％NaCl,3.5％ethanol,4.0％ethanol and pH5 stress(P＜0.001).The results of stress survival test showed that the survival rate of the deletion strain was significantly lower than the wild strain after 1 h treatment with pH3 and 10 mmol/L H2 O2 stress(P＜0.010).The biofilm forming ability of the deletion strain was decreased compared with that of the wild strain(P＜0.050).This study confirmed that lmo2363 gene mediated the adaptation of LM to low temperature,high osmotic pressure,ethanol and acid stress environment and affected the formation of LM bio-film.This study laid a foundation for further exploring the function of lmo2363 gene in the stress resistance process of Listeria monocytogenes.
