Construction of recombinant CVI988 vector vaccine integrating IBDV-VP2 gene
10.16303/j.cnki.1005-4545.2024.09.04
- VernacularTitle:整合IBDV-VP2基因的重组CVI988载体疫苗的构建
- Author:
Lele GONG
1
;
Xinxiang HUANG
;
Yunzhe KANG
;
Lele WANG
;
Xiangqi QIU
;
Yuanyuan ZHANG
;
Meijie GAO
;
Wenhui ZHU
;
Yulin ZHANG
;
Guoqing ZHUANG
;
Aijun SUN
Author Information
1. 河南农业大学动物医学院国家动物免疫学国际联合研究中心,河南郑州 450046
- Keywords:
bacterial artificial chromosome;
Marek's disease virus;
infectious bursal disease virus;
live vector vaccines
- From:
Chinese Journal of Veterinary Science
2024;44(9):1865-1871
- CountryChina
- Language:Chinese
-
Abstract:
The emergence of high virulent mutant strains of infectious bursal disease virus(IBDV)becomes a serious threat to the poultry industry.However,the live attenuated IBDV vaccine can potentially revert to a virulent strain.Therefore,it is a necessary to develop safe and effective IB-DV-associated vaccines.The construction of a recombinant Marek's disease(MD)vaccine strain,CVI988,expressing the IBDV VP2 protein,can protect against disease induced by both IBDV and Marek's disease virus(MDV).Here,the IBDV-VP2 gene was integrated into the UL55 locus of CVI988 by bacterial artificial chromosome(BAC)technique,resulting in the recombinant virus CVI988 BAC-VP2.The recombinant virus was characterized by PCR,IFA and subsequently the bi-ological properties of the recombinant virus were investigated.The results showed that the recom-binant virus CVI988 BAC-VP2 was successfully rescued.The VP2 protein stably expressed in chick-en embryo fibroblasts(CEF).The growth kinetics and plague size assays showed that there was comparable replication ability between recombinant virus and parental virus.This study provides the basis for the development of a low-cost vaccine against both IBDV and MDV infections.
