Isolation,identification,gene sequence analysis and pathogenicity of H3N2 subtype swine influenza virus
10.16303/j.cnki.1005-4545.2024.09.01
- VernacularTitle:1株H3N2亚型猪流感病毒的分离鉴定、基因序列分析及其致病性评价
- Author:
Chaoyang CHEN
1
,
2
;
Yingxue ZHANG
;
Congcong XU
;
Yuanjie SHI
;
Long CAO
;
Xijun YAN
;
Lijie TANG
Author Information
1. 东北农业大学动物医学学院,黑龙江哈尔滨 150030
2. 华威特(江苏)生物制药有限公司,江苏泰州 225300
- Keywords:
swine influenza virus;
H3N2 subtype;
isolation and identification;
sequence analysis;
pathogenicity
- From:
Chinese Journal of Veterinary Science
2024;44(9):1841-1847
- CountryChina
- Language:Chinese
-
Abstract:
The nasal swab samples of swine influenza(SI)suspected pigs were collected and tested for H3 subtype swine influenza virus(SIV)positive by RT-qPCR.The positive samples were inoc-ulated into SPF chicken embryos for virus isolation.The full genome sequencing and sequence anal-ysis of the isolated H3N2 subtype SIV were conducted,and its pathogenicity was studied.The re-sults showed that a strain of SIV was successfully isolated and identified as H3N2 subtype by RT-PCR,named A/Swine/Yunnan/KM/06/2023(H3N2).The BLSAT results showed that the eight segments of SIV H3N2 KM had the highest homology with eight different strains of swine influ-enza or human influenza viruses,reaching 95.41％-97.49％.The HA and NA segments were de-rived from H3N2 subtype SIV,the NP segment was derived from H1N1 subtype human influenza virus,the M segment was derived from H1N2 subtype SIV,and all other segments were derived from H1N1 subtype SIV.The key receptor sites(190D,223V,226I,228S)of HA protein remained unchanged.The pathogenicity experiment results showed that infected piglets exhibited symptoms such as fever,sneezing,runny nose,the virus could be detoxified to the outside through the nasal cavity,and the lungs had different degrees of lesion.Immunohistochemistry(IHC)showed that the virus could replicate in the lungs.In conclusion,a strain of H3N2 subtype SIV was successfully iso-lated,and the genetic evolution,molecular characteristics and pathogenicity of the virus were stud-ied.It revealed that H3N2 subtype SIV is constantly evolving and had pathogenicity to piglets,pro-viding a reference for monitoring and preventing SIV epidemics in China,and provided a candidate strain for SI vaccine development.
