Effects of Coptis detoxification powder on transcription of PBMC TLRs mRNA and its mediated expression of inflammatory factors in Bama pigs under heat stress
10.16303/j.cnki.1005-4545.2024.08.31
- VernacularTitle:黄连解毒散对热应激条件下巴马香猪PBMC TLRs mRNA转录及其介导的炎症因子表达的影响
- Author:
Lin DONG
1
,
2
,
3
;
Yanping WANG
;
Qianqian XU
;
Jishan LIU
;
Jinliang WANG
;
Hualei WANG
;
Zhiqiang SHEN
Author Information
1. 山东省滨州畜牧兽医研究院,山东滨州 256600
2. 山东绿都生物科技有限公司,山东滨州 256600
3. 吉林大学动物医学学院,吉林 长春 130062
- Keywords:
Coptis detoxification powder;
heat stress;
TLRs;
inflammatory factors;
Bama pig
- From:
Chinese Journal of Veterinary Science
2024;44(8):1813-1820
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the effects of Coptis detoxification powder on immune suppression and inflammatory response caused by heat stress in animals,Bama pigs were used as experimental animals to establish an animal model of heat stress,and the effects of Coptis detoxification powder on transcriptional activation of TLRs mRNA in PBMC induced by heat stress and its mediated ex-pression of inflammatory factors were studied in vivo.The animal model of heat stress was estab-lished by artificial climate greenhouse,Chinese medicine"Coptis detoxification powder"was pre-pared,and its inhibitory effect on heat stress and inflammation was studied by preventive and therapeutic administration.Real-time fluorescence quantitative PCR was used to determine the transcriptional changes of HSP70,TLRs and IL-17 mRNA,and ELISA was used to determine the dynamic changes of serum IL-2,IL-6 and TNF-α.Western blot and indirect immunofluorescence(IFA)were used to determine the nuclear translocation and protein expression of p65 phosphoryl-ated protein in NF-κβ inflammatory factor pathway.The transcription of PBMC TLRs mRNA and its mediated inflammatory factor expression and function in Bama pigs under heat stress were sys-tematically analyzed.The results showed that the experimental animal model of Bama pigs under heat stress was successfully established,and heat stress significantly up-regulated the mRNA tran-scription levels of HSP70,TLR2,TLR4 and inflammatory factor IL-17,resulting in significantly enhanced expressions of serum inflammatory factors IL-2,IL-6 and TNF-α.The NF-κβ pathway significantly promoted the level of p65 nuclear phosphorylation and the expression of cytoplasmic protein.The Chinese medicine"Coptis detoxification powder"could significantly reduce the upreg-ulation effect of heat stress conditions on the transcription levels of HSP70 and TLRs mRNA,thus inhibiting the mRNA transcription of inflammatory factor IL-17 and the expression of IL-2,IL-6 and TNF-α in serum,and significantly antagonizing the transcriptional activity of NF-κβ caused by heat stress.It showed good anti-heat stress effect.The anti-inflammatory effects of Chinese veteri-nary medicine prescription"Coptis detoxification powder",its pathway of action and its effectors were investigated,the expression and secretion levels of the regulatory factors related to the path-way associated with TLRs mRNA inhibition were analyzed,and the functional effects and mecha-nism of its clinical anti-heat stress and anti-inflammatory effects were clarified,which laid a data foundation for the promotion and application of this prescription and related mechanism research.
