Study on mechanism of nonylphenol induced oxidative damage and iron death in hepatocytes

Yuxin Wang; Danna Chen; Zhuofan Lei; Yanlu Zou; Changchang Cao; Quanjiang Song; Houhui Song; Sheng Jiang

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Study on mechanism of nonylphenol induced oxidative damage and iron death in hepatocytes

VernacularTitle:壬基酚诱导肝细胞氧化损伤及铁死亡机制研究
Author: Yuxin WANG ^{1
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2
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3
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4
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5} ; Danna CHEN ; Zhuofan LEI ; Yanlu ZOU ; Changchang CAO ; Quanjiang SONG ; Houhui SONG ; Sheng JIANG
Author Information

1. 浙江农林大学动物医学学院,浙江杭州 311300
2. 浙江省畜禽绿色生态健康养殖应用技术研究重点实验室,浙江杭州 311300
3. 动物健康互联网检测技术浙江省工程研究中心,浙江杭州 311300
4. 浙江省动物医学与健康管理国际科技合作基地,浙江杭州 311300
5. 中澳动物健康大数据分析联合实验室,浙江杭州 311300
Keywords: ferroptosis; oxidative stress; nonyplphenol; BRL-3A
From: Chinese Journal of Veterinary Science 2024;44(8):1793-1799
CountryChina
Language:Chinese
Abstract: The aim of this experiment is to investigate the mechanism of liver cell damage induced by nonylphenol(NP).After establishing an in vitro model of NP induced BRL-3A liver cell injury,changes of oxidative damage markers were evaluated,Fe2+content was determined,the ultrastruc-ture of BRL-3A was observed,and the expression level of iron death marker proteins were deter-mined.The results showed that NP could significantly increase ROS and MDA in BRL-3A cells(P＜0.05);the GSH content,GSH Px activity,and SOD activity were significantly reduced(P＜0.05);the Fe2+content significantly increased(P＜0.05)and showed a dose-dependent effect;the mitochondrial volume of BRL-3A cells significantly decreases,the mitochondrial cristae break or e-ven disappear,and some mitochondria show vacuolization;the expression levels of iron homeostasis related proteins TFR1 and FTH1 were significantly reduced(P＜0.05).The results indicate that ferroptosis is involved in the mechanism of NP induced liver cell damage,which bene-fits for further exploration of NP pathogenesis.