Protective effect of glycyrrhiza polysaccharide on H2O2-induced oxidative stress damage in broiler liver cells
10.16303/j.cnki.1005-4545.2024.08.26
- VernacularTitle:甘草多糖对H2O2诱导肉鸡肝细胞氧化应激损伤的保护作用
- Author:
Xueying WANG
1
;
Huining HOU
;
Yongsheng AN
;
Jia LU
;
Rishun LI
;
Cai ZHANG
Author Information
1. 河南科技大学动物科技学院,河南洛阳 471023
- Keywords:
glycyrrhiza polysaccharide;
broiler;
liver cells;
oxidative stress
- From:
Chinese Journal of Veterinary Science
2024;44(8):1773-1781
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to explore the protective effect of glycyrrhiza polysaccha-ride on oxidative stress-induced liver cell damage in broiler chickens,and to study its mechanism of action.Primary broiler liver cells cultured in vitro were divided into blank control group,H2 O2 in-duction group,glycyrrhiza polysaccharide group and H2O2+glycyrrhiza polysaccharide group;CCK-8 reagent was used to detect liver cell activity in broiler chickens,JC-1 and DAPI staining kits were used to detect cell apoptosis,relevant kits were used to detect GSH-Px,SOD,CAT,MDA content,and T-AOC levels.qRT-PCR was used to detect antioxidant genes Nrf2,Keap-1,GPx1,SOD,CAT and inflammatory genes TLR4,MyD88,NF-κB,TNF-α、IL-6,IL-1β、IL-4 and IL-10.The results showed that compared with the H2O2 induced group,glycyrrhiza polysaccharide pretreat-ment could significantly enhance liver cell activity and reduce cell apoptosis in broiler chickens;compared with the H2O2 induced group,glycyrrhiza polysaccharide pretreatment could significant-ly increase cell GSH-Px,SOD,CAT,MDA content and T-AOC levels,and inhibit MDA genera-tion;qRT-PCR detection showed that glycyrrhiza polysaccharide could significantly increase the relative expression levels of Nrf2,Keap-1,GPx1,SOD,CAT,IL-4 and IL-10,while significantly re-ducing MyD88,NF-κB,TNF-α,IL-6 and IL-1β.The results indicated that glycyrrhiza polysaccha-ride enhance the activity of antioxidant enzymes by activating the Nrf2 signaling pathway and in-hibit TLR4 signaling pathway transduction,significantly improving oxidative stress damage in broiler liver cells.
