Detection of Japanese encephalitis virus in livestock in Xinjiang Uygur Autono-mous Region of China from 2021 to 2022
10.16303/j.cnki.1005-4545.2024.08.18
- VernacularTitle:2021-2022年我国新疆维吾尔自治区家畜流行性乙型脑炎病毒检测
- Author:
Bing LI
1
,
2
;
Yan GAO
;
Xinyu CAO
;
Xiangshu QIU
;
Aijian QIN
;
He ZHANG
;
Ningyi JIN
Author Information
1. 扬州大学兽医学院江苏省动物预防医学重点实验室,江苏扬州 225001
2. 中国农业科学院长春兽医研究所,吉林长春 130117
- Keywords:
Japanese encephalitis virus;
epidemiology;
E gene;
test method
- From:
Chinese Journal of Veterinary Science
2024;44(8):1713-1718
- CountryChina
- Language:Chinese
-
Abstract:
The SYBR Green Ⅰ real-time fluorescence quantitative PCR detection method was used to determine the prevalence of Japanese encephalitis virus(JEV)in mosquito vectors and cattle se-rum samples in Xinjiang.The E gene fragment of the JEV strain was amplified by PCR,cloned into a pEASY-Blunt vector,produced as a recombinant plasmid,and its sensitivity,specificity and re-producibility were verified.Between 2021 and 2022,serum samples were taken in the regions of Hami,Altay,Ili,Aksu,and Kashi in order to monitor the prevalence of JEV in livestock in Xin-jiang.The positive rate was discovered and evaluated using the established detection method.The established detection method showed a good linear relationship,and the detection interval was 4.03X102-4.03×109 copies/pL.The correlation coefficient was 0.995,the slope was-3.431,and the extreme value of the lower limit of sensitivity was 4.03 × 102 copies/pL.This method has no specific amplification for Zika virus(ZIKV)and Dengue virus(DENV).The intra group coefficient of variation of reproducibility was 0.53％-1.27％,and the inter group coefficient of variation was 0.48％-1.43％.Using this method to detect serum samples from livestock in Xinjiang from 2021 to 2022,the total positive rate was 3.28％,with positive detection rates in horses,cows,and sheep being 2.35％,6.77％,and 3.74％respectively,the virus was identified as Type Ⅰ JEV.A SYBR Green Ⅰ real-time PCR method for the detection of genotype 1 JEV was established.JEV was de-tected in the serum of horses,cattle and sheep in Xinjiang,with a total positive rate of 3.11％.
