A visual RT-LAMP-VF method for detection of Zika virus nucleic acid
10.16303/j.cnki.1005-4545.2024.08.16
- VernacularTitle:检测寨卡病毒核酸的RT-LAMP可视化方法的建立
- Author:
Yumeng SONG
1
;
Pei HUANG
;
Hongli JIN
;
Cuicui JIAO
;
Yujie BAI
;
Mengyao ZHANG
;
Zhiyuan GONG
;
Yuanyuan LI
;
Haili ZHANG
;
Hualei WANG
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/教育部人兽共患病研究重点实验室,吉林长春 130062
- Keywords:
Zika virus;
RT-LAMP;
immunochromatography;
nucleic acid test
- From:
Chinese Journal of Veterinary Science
2024;44(8):1698-1703
- CountryChina
- Language:Chinese
-
Abstract:
To establish a simple,convenient,sensitive,and specific method for rapid detection of Zi-ka virus(ZIKV),the whole genome sequences of ZIKV isolated from different times and regions were analyzed.The specific primers and probes were designed based on the screened target se-quences located in the conserved region of the ZIKV NS5 gene.By combining RT-LAMP isother-mal amplification technology and immunochromatography technology,a reverse transcription loop mediated isothermal amplification nucleic acid and flow visualization strip(RT-LAMP-VF)detec-tion method for ZIKV was established.The results showed that the method had good specificity and sensitivity.When the ratio of inner,outer,and ring primers(FIP∶LF∶F3)was 4∶2∶1,the detection method can specifically detect 102 copies/pL RNA transcripts or 2.15 pfu ZIKV at 61 ℃for 45 minutes,with no cross reaction with other flaviviruses such as Japanese encephalitis virus and classical swine fever virus.Other RNAs in blood tissue samples did not affect the sensitivity and specificity of RT-LAMP-VF,indicating that the method can be applied to clinical practice.The ZIKV RT-LAMP-VF detection method established in this study is easy to perform and does not require special instruments and equipment.It is particularly suitable for the rapid detection of ZIKV in grassroots units,providing technical support and material support for the establishment of on-site rapid detection and early warning and prediction systems for ZIKV disease.
