Isolation,identification and characterization of a virulent phage of Clostridium perfringens
10.16303/j.cnki.1005-4545.2024.08.15
- VernacularTitle:一株产气荚膜梭菌烈性噬菌体的分离鉴定及特性分析
- Author:
Mengjiao LI
1
;
Zhanyun SONG
;
Bo LIU
;
Zhiqiang XU
;
Yue LIU
;
Qiulin WANG
;
Xin FENG
Author Information
1. 吉林大学动物医学学院人兽共患病研究教育部重点实验室,吉林长春 130062
- Keywords:
Clostridium perfringens;
biological characteristics of phage;
genome
- From:
Chinese Journal of Veterinary Science
2024;44(8):1691-1697
- CountryChina
- Language:Chinese
-
Abstract:
Clostridium perfringens is a common Gram-positive anaerobic conditioned pathogen,widely existing in nature,which can cause diarrhea,gas gangrene,and other diseases.Antibiotics are used in the clinical treatment of Clostridium perfringens infection,but the bacteria will devel-op resistance through mutation,drug-resistant plasmid transmission,and other ways,so that Clos-tridium perfringens can survive under the environmental pressure of antibiotics.Therefore,it is very important to find and develop new preparations to replace antibiotics or as feed additives to target the removal of Clostridium perfringens from the body or to prevent infection.In this study,a virulent Clostridium perfringens phage vB_CPP_AT was isolated from sewage by double plate method.The morphology of the bacteriophage was observed by transmission electron microscope.The biological characteristics of the bacteriophage were analyzed by lytic spectrum,MOI,pH,and temperature tolerance.The results showed that the vB_CPP_AT belongs to the Podoviridae.It would grow explosively at 60 min with an optimal MOI of 0.1.The vB_CPP_AT only lyse Clos-tridium perfringens and the lytic rate was 40％(8/20).No cleavage reaction occurred with other bacteria tested.The phage had good thermal stability and acid-base tolerance.Genomic analysis re-vealed that the phage had double-stranded DNA with a total length of 16 790 bp,and 20 open read-ing frames.Genomic analysis of vBCPPAT showed that it was a new virulent phage of Clostridi-um perfringens.The results laid a foundation for the clinical treatment of Clostridium perfringens with phage.
