Differential expression profile in Neuro-2a cells infected by rabies virus
10.16303/j.cnki.1005-4545.2024.08.14
- VernacularTitle:狂犬病病毒感染N2a细胞引起的转录体差异表达
- Author:
Xiaomin LIU
1
;
Yidi GUO
;
Xin GUO
;
Yannan ZHANG
;
Chongyang WANG
;
Zichen WANG
;
Danwei ZHANG
;
Maolin ZHANG
Author Information
1. 吉林大学动物医学学院人兽共患病研究教育部重点实验室,吉林长春 130062
- Keywords:
rabies virus;
N2a cell;
nervous system;
transcriptome
- From:
Chinese Journal of Veterinary Science
2024;44(8):1682-1690
- CountryChina
- Language:Chinese
-
Abstract:
Rabies is a zoonotic disease that poses a global public health threat.Rabies virus(RABV)is neurotropic and can cause severe neurological disorders and behavioral abnormalities in host,with a fatality rate nearly 100％.In order to identify the key genes for RABV affecting neuronal cell function,we established and analyzed the mRNA expression profile of Neuro-2a(N2a)cell in-fected with challenge virus standard(CVS)-11 by RNA sequencing(RNA-seq).Biological func-tions of differentially expressed genes(DEGs)were determined by GO and KEGG enrichment a-nalysis.The results showed that there were 415 differentially expressed genes in N2a infected with CVS-11 strain,of which 89 were up-regulated and 326 were down-regulated.These genes were re-lated to a variety of biological processes,such as axon guidance pathway,cholesterol metabolism pathway,nitrogen metabolism pathway,and glycosphingolipid biosynthesis pathway,many of them have been shown to be closely associated with RABV infection.A total of 12 DEGs related to axon conduction,antigen processing and presentation pathways were selected and detected by real-time PCR,and their expression trends were consistent with the RNA-seq results.The genomic tran-scriptomic data on N2a cell under RABV infection will provide new clues for probing the mecha-nisms of RABV infection and transmission in the nervous system in the future.
