Wholegenome sequencing and function analysis of Ligilactobacillus salivarius CICC23174
10.16303/j.cnki.1005-4545.2024.08.11
- VernacularTitle:唾液乳杆菌CICC23174全基因组测序及功能分析
- Author:
Liping WANG
1
;
Lingcong DENG
;
Dahong WANG
;
Maopeng WANG
;
Gefen YIN
Author Information
1. 云南农业大学动物医学院,云南昆明 650201
- Keywords:
Ligilactobacillus salivarius;
genomics;
bioinformatics;
functional annotations;
bacterio-cin;
gene collinearity analysis
- From:
Chinese Journal of Veterinary Science
2024;44(8):1659-1666
- CountryChina
- Language:Chinese
-
Abstract:
To study the biological characteristics and gene function of Ligilactobacillus salivarius CICC23174 strain,a strain isolated from chicken intestines.The next generation sequencing plat-form HiSeq2000 was used for sequencing,and bioinformatics software was employed to assemble and optimize the raw data.Functional annotation,bacteriocin and signal peptide,gene collinearity comparison,and phylogenetic analysis were performed on its gene information.Our results indica-ted that the linear genome of Ligilactobacillus salivarius CICC23174 was 203 542 bp in length,with a GC content of 32.84％,encoding 1 890 genes,containing 3 bacteriocins,and 50 signal pep-tides.Importantly,the Ligilactobacillus salivarius CICC23174 included into 27 unique genes,forming four systems:the Type Ⅰ CRISPR Cas defense system,the Type Ⅱ toxin antitoxin system,the tumor escaped inhibitory gene,and the protein secretion pathway.Moreover,collinearity analy-sis showed that CICC23174 strain was most similar to the Ligilactobacillus salivarius UCC118,but 16S rRNA phylogenetic analysis indicted that CICC23174 strain was genetically close to FZJTZ9M6 strain.The results of this study lay the foundation for enriching the species evolution-ary library of Lactobacillus salivarius and exploring the potential probiotic functions of Lactoba-cillus salivarius.
