Inhibition of PCV2 on IL-15 in inguinal lymph nodes of piglets
10.16303/j.cnki.1005-4545.2024.08.03
- VernacularTitle:PCV2对仔猪腹股沟淋巴结内IL-15的抑制作用
- Author:
Yanan ZHANG
1
;
Feiyan WANG
;
Chen YUAN
;
Jing REN
;
Kai SU
;
Huaining YUE
;
Shuanghai ZHOU
;
Huanrong LI
;
Qinye SONG
Author Information
1. 河北农业大学动物医学院河北省兽医生物技术创新中心,河北保定 071000
- Keywords:
porcine circovirus type 2;
piglets;
inguinal lymph nodes;
IL-15
- From:
Chinese Journal of Veterinary Science
2024;44(8):1593-1599,1621
- CountryChina
- Language:Chinese
-
Abstract:
Porcine circovirus type 2(PCV2)mainly damages the immune cells of pigs,causing lym-phocyte depletion and immune suppression.Interleukin(IL)-15 regulates immune functions wide-ly,and plays an important regulatory role in the survival,proliferation,differentiation and immune function of a variety of immune cells such as natural killer(NK),CD8+T cells and NKT cells.In this study,in order to determine the effect of PCV2 on IL-15 expression,4-week-old piglets(n=4)were infected with PCV2 and the negative control group(n=4)was set up.On day 7 post-infec-tion,the inguinal lymph nodes of the infected and control groups were collected,and porcine cyto-kine antibody microarray(QAP-CYT-1)was employed to quantify the expression of cytokines in the tissues,screen for differential cytokines,and GO and KEGG enrichment analysis for IL-15 were conducted.Real-time quantitative fluorescent PCR(qPCR)and ELISA were used to verify the level of IL-15 mRNA and protein,and those in porcine peripheral blood mononuclear cells(PBMC)and serum were simultaneously detected.Compared with the negative control group,the expression lev-el of IL-15 was significantly reduced in the infected group(P＜0.05);IL-15 was mainly involved in cytokine-cytokine receptor interactions,immune responses,cellular activation,and the regulation of JAK-STAT and TNF signaling pathways.The levels of IL-15 mRNA and protein in inguinal lymph nodes in the infected group were significantly lower than those in the control group(P＜0.05),which was consistent with the detection results of QAP-CYT-1.However,there was no significant difference in IL-15 mRNA and protein levels in PBMC and serum.These results indicate that PCV2 can inhibit IL-15 in the inguinal lymph node microenvironment of piglets.This study can provide important information for further revealing the immunosuppressive mechanism of PCV2.
