Effects of chronic poisoning by As2O3 in drinking water on TRβ1-mediated thyroid hormone signaling pathway in rat liver
10.16303/j.cnki.1005-4545.2024.07.24
- VernacularTitle:As2O3饮水慢性中毒对大鼠肝脏TRβ1介导的甲状腺激素信号通路的影响
- Author:
Lian WANG
1
,
2
;
Xuefei WANG
;
Kui GAO
;
Yingchun GUO
;
Tianyang ZHANG
;
Ying YANG
Author Information
1. 河南牧业经济学院动物医药学院,河南郑州 450000
2. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
- Keywords:
As2O3;
hepatotoxicity;
TH signal pathway;
TRβ1;
rat
- From:
Chinese Journal of Veterinary Science
2024;44(7):1526-1533,1578
- CountryChina
- Language:Chinese
-
Abstract:
Previous studies have found that As2 O3 can interfere with serum thyroid hormone TH levels in rats and cause chronic liver damage,but the mechanism remains unclear.In order to ex-plore the role of TH signaling pathway in As2 O3-induced chronic liver injury,qRT-PCR and West-ern blot techniques were used to detect the expression changes of genes and protein of TRβ1(a key regulator of TH signaling pathway in rat liver)and cyclin D1(the downstream factor of TRβ1 in nuclear pathway).Meanwhile,the changes in the protein of key factors(Bax,Bcl-2)of the TH sig-nal nuclear outside pathway were detected.The results indicated that:after As2 O3 treatment for 110 days,compared with the control group,the expression of TRβ1 protein in the liver of female mice significantly decreased(P＜0.01),the expression of cyclin D1 significantly increased in the 0.1 and 0.2 mg/L groups(P＜0.01).Meanwhile,the expression of TRβ1 protein in male mice sig-nificantly decreased in 0.4 mg/L group(P＜0.01),and the expression of cyclin D1 in each group significantly increased(P＜0.01).The mRNA expression results were basically the same as those of protein expression.After As2 O3 treatment for 194 days,compared with the control group,the expression of TRβ1 protein in each group significantly decreased(P＜0.01),and the expression of cyclin D1 significantly increased(P＜0.01).The mRNA expression results were basically consist-ent with the protein.As2 O3 interfered with the expression of Bcl-2 and Bax proteins in rats and in-duced the increase in the ratio of Bcl-2/Bax protein as the action time increased.Among them,the Bcl-2/Bax ratio of female rats in each group and male rats in the 0.4 mg/L group significantly in-creased(P＜0.01),and male rats in the 0.1 mg/L group significantly increased(P＜0.05).It shows that As2O3 can cause abnormal levels of TRβ1,cyclin D1 and the Bcl-2/Bax ratio in rat liv-er.
