Projection pathway of VGlut2 neurons from paraventricular nucleus
10.16303/j.cnki.1005-4545.2024.07.22
- VernacularTitle:室旁核VGlut2神经元投射通路
- Author:
Xing WANG
1
,
2
;
Haodong LIU
;
Penghui LI
;
Jiacheng LI
;
Qi FAN
;
Rui YAN
;
Yang HE
;
Ming ZHANG
;
Xin ZHOU
;
Chenguang DU
Author Information
1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018
2. 内蒙古自治区基础兽医学重点实验室,内蒙古呼和浩特 010018
- Keywords:
hypothalamic paraventricular nucleus;
glutamatergic neurons;
nerve projection;
energy metabolism;
stereotactic brain injection;
mice
- From:
Chinese Journal of Veterinary Science
2024;44(7):1514-1520
- CountryChina
- Language:Chinese
-
Abstract:
Vesicular glutamate transporter 2(VGlut2)is expressed in the PVN of the hypothalamic paraventricular nucleus(PVNVG1ut2)as an excitatory neurotransmitter,which regulates food intake and energy metabolism and plays an important role in maintaining homeostasis.However,it is not clear that the upstream and downstream projection network of PVNVGut2 neurons hinders the anal-ysis of glutamatergic neuron circuit function.Anterograde and retrograde tracer viruses were injec-ted into the PVN of VGlut2 mice by stereotactic brain injection technique to find the input and output nuclei of PVNVGlut2 neurons.Anterograde tracing results showed that PVNVGlut2 neurons pro-jected to the downstream medial amygdala(MeAD)and arcuate nucleus(ARC).Retrograde trac-ing results showed that PVNVGlut2 received input from the prefrontal nucleus(Pr),the reticular tegmental nucleus(RtTg),and the hypoglossal nucleus(12N).In addition,VGlut2 was found to be co-expressed with neuronal nitric oxide synthase(nNOS)neurons in the PVN.The anatomical net-work of PVNVG1ut2 neurons was analyzed by virus tracking tool,which laid the anatomical founda-tion for further study on the functional regulation of PVNVGlut2.
