Dahuang Fuzi decoction enhances killing effect of NK cells on Lewis lung carcino-ma cells
10.16303/j.cnki.1005-4545.2024.07.18
- VernacularTitle:大黄附子汤增强NK细胞杀伤小鼠LLC细胞效力
- Author:
Xiaoyan WANG
1
;
Mingxuan WANG
;
Jiaxuan FENG
;
Jinping BAI
;
Bingmei WANG
;
Chang LIU
Author Information
1. 长春中医药大学临床医学学院,吉林长春 130021
- Keywords:
Dahuang Fuzi decoction;
NK cells;
lung cancer;
MIC A/B;
mice
- From:
Chinese Journal of Veterinary Science
2024;44(7):1483-1488
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the effect of Dahuang Fuzi decoction(DHFZT)on the killing effect of natural killer(NK)cells and the growth of lung cancer xenografts in mice.Lewis lung cancer xenograft model was constructed,and the mice were randomly divided to the normal saline group,low and high concentrations of DHFZT groups.The growth of lung cancer xenografts in mice in the control group,low and high concentration of DHFZT treatment groups was detected.The levels of IFN-y,IL-2 and IL-10 were detected by ELISA.The killing effect of NK cells was detected by calcein release assay.The release of CD107α was detected by flow cytometry.The expression of activated receptors and the tumor cell surface ligands were detected in the con-trol group and DHFZT treatment groups.The results showed that the xenografts of Lewis mice in the DHFZT treatment groups were grown slower than that in the control group in a dose depend-ent manner(P＜0.05).Compared with the control groups,the levels of IL-2 and IFN-y in tumor tissues were increased in DHFZT treatment groups(P＜0.05),but no significant changes in IL-10.Calcein release assay showed that the killing efficiency of NK cells in the DHFZT treatment groups was increased(P＜0.05).The secretion level of IFN-y in the culture supernatant was increased by DHFZT treatment(P＜0.05).DHFZT treatment increased the expression level of CD107α of NK cells.The expression of MIC A/B on the surface of A549 and H1299 cells was up-regulated by DHFZT treatment,but not the expression of inhibitory ligand HLA-ABC.The results showed that DHFZT enhanced the killing effect of NK cells on mouse lung cancer cells by up-reg-ulating the expression of MIC A/B,increasing the secretion of IFN-y and CD107α.Hence,these re-sults indicated that DHFZT suppresses the lung cancer growth potential through regulating the killing effect of NK cells.
