Synthesis and antifungal activity of albumen-derived nano-FeS against Nosema ceranae
10.16303/j.cnki.1005-4545.2024.07.11
- VernacularTitle:蛋清源nano-FeS的合成及对东方蜜蜂微孢子虫的抗菌活性
- Author:
Haodong WU
1
;
Hao SUN
;
Le CHEN
;
Can HUANG
;
Shang CHENG
;
Lijing CAO
;
Qinghua WANG
Author Information
1. 西南大学动物医学院,重庆荣昌 402460
- Keywords:
nano-FeS;
microsporidia;
isolation and identification;
antifungal activity;
ferroptosis
- From:
Chinese Journal of Veterinary Science
2024;44(7):1418-1429
- CountryChina
- Language:Chinese
-
Abstract:
Based on the isolation and identification of Nosema ceranae(N.ceranae)in honeybees,this study optimized the synthesis method of nano-FeS derived from albumen and explored in vitro and in vivo antifungal effects against N.ceranae.Pathogens were isolated from infected honeybee colonies and identified as N.ceranae using morphological and molecular biology techniques.In vitro experiments were conducted to confirm the antifungal effects of nano-FeS against N.ceranae and elucidate the mechanism.In vivo experiments were carried out to validate the therapeutic effects of nano-FeS against N.ceranae infection.Nano-FeS was synthesized using the solvothermal method with an optimal scheme determined through orthogonal experiments,with an average par-ticle size of 75 nm.Flow cytometry and fluorescence staining experiments confirmed that nano-FeS induced apoptosis and necrosis in N.ceranae.After N.ceranae was exposed to nano-FeS,intracellu-lar iron accumulation,disruption of the glutathione and glutathione peroxidase antioxidant system,and subsequent ROS accumulation were observed,ultimately leading to lipid peroxidation of cell membranes.In vivo experiments demonstrated reduced mortality and decreased spore counts in the midgut of honeybees fed with nano-FeS.Transcriptome analysis and qPCR revealed the impact of nano-FeS on gene expression in the N.ceranae infected honeybee midgut.This study presented a promising alternative antifungal agent for N.ceranae infection in honeybees and elucidated the an-tifungal mechanism of nano-FeS related to ferroptosis.Additionally,the study found a positive cor-relation between the mass concentration of nano-FeS and its antifungal effectiveness against N.ceranae.
