Isolation,identification and whole genome sequence analysis of goose astrovirus from Xinjiang
10.16303/j.cnki.1005-4545.2024.07.09
- VernacularTitle:新疆地区鹅星状病毒的分离鉴定及全基因组序列分析
- Author:
Haixia XIAO
1
;
Ling ZHANG
;
Yan WANG
;
Wanpeng MA
;
ASIYEMU·Yasen
;
Jin GAO
;
Zhanqiang SU
Author Information
1. 新疆农业大学动物医学学院,新疆乌鲁木齐 830025
- Keywords:
goose astrovirus;
isolation and identification;
whole genome sequencing;
homology;
ge-netic evolution
- From:
Chinese Journal of Veterinary Science
2024;44(7):1401-1407
- CountryChina
- Language:Chinese
-
Abstract:
In order to understand the infection and molecular genetic characteristics of goose astro-virus(GAstV)in Hotan,Xinjiang,visceral organs and swabs of dead goslings were collected asep-tically from three goose farms in Hotan,Yutian and Pashan counties,and GAstV was detected by RT-PCR.The positive samples were screened and identified in LMH cells,and the whole genome was sequenced,and the genetic characteristics of the isolates were analyzed.The results showed that the total positive rate of GAstV was 11.25％(65/578).Two strains of GAstV named as GAstV/XJHT-1 and GAstV/XJHT-2 were isolated and the lengths of their genome sequences were determined as 7 190 bp and 7 125 bp,respectively.Whole genome homology analysis showed that the homology of the two isolates with GAstV-1 and GAstV-2 was higher than 95％,and the homology with other sources(chicken,duck,and turkey)ranged from 54.1％to 61.5％.Genetic e-volution analysis showed that the genetic distance between GAstV isolates from Henan and Anhui was relatively close,suggesting that the isolated GAstV may be related to the introduction of gos-lings or goose eggs from these two places.The findings provide a basis for further development of vaccines or control products.
