Ghrelin affects feed intake and body weight of mice through CART neurons in lat-eral hypothalamic nucleus
10.16303/j.cnki.1005-4545.2024.06.22
- VernacularTitle:Ghrelin通过下丘脑外侧核CART神经元影响小鼠采食和体质量
- Author:
Xiaojuan CAO
1
;
Haodong LIU
;
Penghui LI
;
Jiacheng LI
;
Qi FAN
;
Xing WANG
;
Yu-Jie CHEN
;
Rihan HAI
;
Xiaoyu ZHANG
;
Chenguang DU
Author Information
1. 内蒙古农业大学职业技术学院,内蒙古包头 014109
- Keywords:
amylin;
glutamatergic neurons;
melanocortin type 4 receptor;
paraventricular nucleus
- From:
Chinese Journal of Veterinary Science
2024;44(6):1268-1273
- CountryChina
- Language:Chinese
-
Abstract:
Ghrelin is a hormone produced by the stomach that regulates energy metabolism after acting on the central nervous system.Cocaine amphetamine-regulated transcriptional peptide(CART)neurons participate in the regulation of feeding behavior and energy balance.It is known that CART neurons are influenced by hormones to regulate energy homeostasis,but whether ghre-lin exerts its pro-appetite function by influencing CART neurons is unknown.Therefore,this study focuses on the role of VMHCART neurons in the regulation of feeding and relative body weight by ghrelin.Firstly,the whole brain expression of CART was determined by immunofluorescence.Then the effect of intraperitoneal injection of ghrelin on the expression of DMHCART neurons was evalua-ted.Finally,the ghrelin was delivered to DMH and the changes of food intake and relative body weight of mice were measured.CART immunoreactive neurons were detected in medial preoptic nucleus(MPA),arcuate nucleus(ARC),dorsomedial hypothalamic nucleus(DMH),thalamic pa-raventricular nucleus(PVT)and raphe nucleus(ROb).Compared with the control group,periph-eral injection of ghrelin significantly increased the expression of DMHC ART immunoreactive neurons(P=0.037 3).DMH long-term injection of ghrelin resulted in an increase in body weight(P=0.004 0)and feed intake(P=0.023 1).The results provide anatomical evidence for the whole brain distribution of CART,which proves that ghrelin affects feed intake and body weight of mice through CART neurons in DMH,suggesting that specific neuron types and regional specificity are involved in ghrelin regulation of feed intake and energy homeostasis.
