Construction and characterization of monoclonal antibodies against the native H11 protein of Haemonchus contortus
10.16303/j.cnki.1005-4545.2024.06.15
- VernacularTitle:抗捻转血矛线虫天然H11蛋白单克隆抗体的制备及特性分析
- Author:
Feng LIU
1
;
Simin WU
;
Yao ZHANG
;
Shusen LIAO
;
Liurong FANG
;
Min HU
;
Chunqun WANG
Author Information
1. 华中农业大学动物医学院农业微生物资源发掘与利用全国重点实验室,湖北武汉 430070
- Keywords:
Haemonchus contortus;
H11 protein;
monoclonal antibody;
growth
- From:
Chinese Journal of Veterinary Science
2024;44(6):1204-1212
- CountryChina
- Language:Chinese
-
Abstract:
To construct monoclonal antibodies against Haemonchus contortus native H11 protein.In this study,five 4-6 weeks female BALB/c mice were immunized with native H11 protein extrac-ted from adult worms by Concanavalin A lectin.Spleen cells were isolated and fused with SP2/0 cells after 3 times of immunization.Two hybridoma cell lines,named A1E3 and A10E1,which could stably secrete monoclonal antibodies against H11 protein were obtained.The subtype identi-fication and immunological analysis showed that the heavy chain of the two monoclonal antibodies belonged to IgG1 and the light chain was κ type,and both monoclonal antibodies recognized the natural antigen H11.Immunohistochemical localization and larval developmental inhibition test in vitro showed that the mAb A1E3 could be localized to the intestinal microvilli of the adult worm,and that the antibody can inhibit the growth of the fourth-stage larvae.The successful production of two monoclonal antibodies not only lays the foundation for the study of protective antigenic epitopes of the H11 protein and the development of epitope vaccines,but also provides a potential application of the monoclonal antibody for the treatment of haemonchusis in animals.
