Biological characteristics and whole-genome sequencing analysis of a strain of sheep-derived Streptococcus equi
10.16303/j.cnki.1005-4545.2024.06.09
- VernacularTitle:1株羊源马链球菌的生物学特性及全基因组测序分析
- Author:
Hongcai MA
1
;
Jiangyong ZENG
;
Zhenjie YUAN
;
Jia WANG
;
Dongjing WANG
Author Information
1. 西藏自治区农牧科学院畜牧兽医研究所,西藏拉萨 850009
- Keywords:
Streptococcus equi;
sheep;
biological characteristics;
whole genome;
drug resistance test
- From:
Chinese Journal of Veterinary Science
2024;44(6):1148-1155
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the biological characteristics and whole-genome sequence of Streptococ-cus equi,a sheep-derived Streptococcus equi preserved in our laboratory was subjected to recovery,biochemical experiments,drug susceptibility tests and animal experiments,and followed by whole genome sequencing and annotation of the gene functions of the genome by using the biogenetic da-tabase.The biochemical identification results showed that this strain could ferment sugars,but the results of nitrate,catalase,V-P test,and M-R test were negative;the drug susceptibility test results showed that this strain was highly sensitive to most antibiotics and was resistant to kanaphylaxis.It was resistant to amikacin and amikacin;animal experiments showed that the lethality rate of this strain to mice was 100％,and the median lethal dose of this strain was measured to be 4.86X 106 CFU/kg.According to the pathological section results of mice,it showed that the lungs,liver,kidneys,and spleen all had varying degrees of lesions.The whole genome sequencing results showed that the total genome length of this strain is 2 272 497 bp,the G+C content was 41.1％,and it was predicted to contain 2 124 CDS regions.The RNA prediction results showed that the number of rRNA and tRNA was 15,and the number of tRNA was 57.There were four prophages and gene islands,and there were 362 pathogenicity-related genes in the VFDB database without CRISPR sequences.This study analyzed the complete genome of Streptococcus equi derived from sheep,and also provided a theoretical basis for the treatment,prevention and control of the disease.
