Establishment and optimization of a method for transferring golden hamster embryos using true pregnancy recipients
10.3969/j.issn.1671-7856.2024.10.009
- VernacularTitle:真孕受体用于金黄仓鼠胚胎移植的方法建立与优化
- Author:
Yana LAI
1
;
Wentao ZENG
Author Information
1. 南京医科大学医药实验动物中心,南京 211166
- Keywords:
golden hamster;
fertilized egg;
two-cell embryos;
embryo transfer
- From:
Chinese Journal of Comparative Medicine
2024;34(10):72-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the technical parameters used for embryo transfer in golden hamsters,and explore the optimal number and developmental period of transplanted embryos for golden hamster pregnancy recipients.Methods We established a population of true pregnant albino golden hamster embryo transfer recipients and compared the effects of different embryonic developmental stages,recipient embryo reduction,and secondary transfer recipients on litter yield,donor embryo yield,and offspring survival rate.Results Compared with wild-type golden hamster transplant recipients,true pregnancy albino recipients allowed the origin of the offspring to be determined quickly,and were suitable for various reproductive experimental programs based on embryo transfer.The use of fertilized eggs or two-cell embryos had no significant impact on the transfer effect(P＞0.05);however,the rate of donor embryos was significantly increased in the recipient embryo-reduction group(22％,P＜0.05).The second transfer recipient's non-pregnancy rate was significantly increased(42％,P＜0.01).The highest embryo yield rate(27％,P＜0.01)and normal survival rate(89％)occurred with the transfer of 6～10 embryos.Conclusions The transfer of 6～10 donor embryos may improve the yield and survival rate of donor embryos.Here,we successfully established an embryo transfer method using pregnant albino golden hamsters as recipients,thus providing technical support for the application and development of gene modification models in golden hamsters.
