MiR-22-3p targets gasdermin D to inhibit homocysteine-induced pyroptosis of vascular smooth muscle cells
10.3969/j.issn.1671-7856.2024.09.002
- VernacularTitle:miR-22-3p靶向GSDMD抑制同型半胱氨酸诱导的平滑肌细胞焦亡
- Author:
Yingyi ZHONG
1
;
Ning DING
;
Yichen WANG
;
Chao LIU
;
Zhifeng DONG
;
Shengchao MA
;
Jiantuan XIONG
;
Yinju HAO
;
Zhigang BAI
;
Yideng JIANG
Author Information
1. 国家卫生健康委代谢性心血管疾病研究重点实验室,银川 750004
- Keywords:
atherosclerosis;
VSMC;
pyroptosis;
miR-22-3p;
homocysteine
- From:
Chinese Journal of Comparative Medicine
2024;34(9):12-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-22-3p on pyroptosis of vascular smooth muscle cells(VSMCs)induced by homocysteine(Hcy).Methods Human VSMCs were cultured in vitro and divided into a Control group(0 μmol/L Hey)and a Hey group(100 μmol/L Hey).After intervention,expression levels of pro Caspase-1,gasdermin D(GSDMD),N-GSDMD,and NLR family pyrin domain containing 3(NLRP3)were detected by Western blot.MiR-22-3p expression was determined by quantitative real-time reverse-transcription polymerase chain reaction.Interleukin(IL)-1 β and IL-18 levels in the supernatant were measured by enzyme-linked immunosorbent assay.Cells were also transfected with control miR-22-3p(miR-22-3p-NC),miR-22-3p-mimic,and miR-22-3p-inhibitor,to observe the effects on VSMC pyroptosis induced by Hcy.Results Expression levels of pro Caspase-1,GSDMD,N-GSDMD,and NLRP3 in VSMCs were increased(P＜0.05),IL-1 β and IL-18 levels were increased(P＜0.01),and the relative expression level of miR-22-3p was reduced(P＜0.01)in the Hcy group compared with the Control group.Transfection with miR-22-3p-mimic significantly decreased the expression levels of pro Caspase-1,GSDMD,N-GSDMD,and NLRP3 in VSMCs(P＜0.01),and significantly decreased levels of IL-1β and IL-18(P＜0.01),while transfection with miR-22-3p-inhibitor significantly increased the expression levels of pro Caspase-1,GSDMD,N-GSDMD,and NLRP3 in VSMCs(P＜0.01)and significantly increased the levels of IL-1β and IL-18(P＜0.05).Conclusions MiR-22-3p may delay Hcy-induced VSMC pyroptosis.
