Method and improvement of implantable osmotic pump for intraventricular drug administration in rats
10.3969/j.issn.1671-7856.2024.07.010
- VernacularTitle:应用植入式缓释泵实现大鼠脑室给药的操作方法及其改进
- Author:
Heyong SUN
1
;
Gangli ZHANG
;
Jiliang WU
;
Peili CAO
;
Shuo LI
;
Haoqin SUN
Author Information
1. 山西医科大学第五临床医学院神经外科,太原 030012
- Keywords:
implantable osmotic pump;
intraventricular drug administration;
brain stereotaxic apparatus;
experimental method;
technology improvement
- From:
Chinese Journal of Comparative Medicine
2024;34(7):89-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To introduce and enhance an experimental technique for intraventricular drug delivery via an implantable osmotic pump.Methods Eight-week-old male SD rats were selected and the requisite equipment and reagents were prepared.The osmotic pump was assembled and brought to operational status before conducting the implantation surgery.Following anesthesia,the rats underwent skin preparation and the upper surface of the skull was surgically exposed.A point directly above the ventricle was located using a brain stereotaxic apparatus,and a small hole was drilled at that location with a high-speed cranial drill.The pump body was then implanted subcutaneously in the neck and the needle was inserted into the drilled hole,and secured with dental cement.Once solidified,the needle base was removed,the subcutaneous soft tissue and scalp were sutured in layers,and the animal was returned to its cage for rearing in isolation.Results The osmotic pump was successfully implanted subcutaneously in the rat's neck,the needle was securely fixed to the skull,and the catheter interface remained intact.The rats were sacrificed and the brain tissue was removed.Examination of the extracted brain tissue revealed no significant hematoma around the puncture site or needle tract,and the presence of blue dye in the ventricular and adjacent tissues indicated successful drug delivery to the ventricle.Conclusions The introduction of a brain stereotaxic apparatus to aid localization,coupled with enhancements to the operational procedure,may improve the accuracy and safety of the implantation process resulting in a high success rate for intraventricular drug administration.
