Effects and comparison of ginsenosides Rg1 and Rb1 in depression-and anxiety-like behaviors induced by chronic unpredictable stress in rats
10.3969/j.issn.1671-7856.2024.07.008
- VernacularTitle:人参皂苷Rg1和Rb1改善慢性不可预测应激致大鼠抑郁、焦虑样行为的作用比较
- Author:
Xueyi BEI
1
,
2
,
3
;
Ning JIANG
;
Caihong YAO
;
Yiwen ZHANG
;
Xinran SUN
;
Yanqin LUO
;
Liang LI
;
Mengzhou XIE
;
Xinmin LIU
Author Information
1. 湖南中医药大学,长沙 410208
2. 湖南中医药大学湖南省药食同源功能性食品工程技术研究中心,长沙 410208
3. 湖南中医药大学中医心肺病证辨证与药膳食疗重点研究室,长沙 410208
- Keywords:
ginsenoside Rg1;
ginsenoside Rb1;
chronic unpredictable stress model;
inflammatory factor;
depression;
anxiety
- From:
Chinese Journal of Comparative Medicine
2024;34(7):68-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of ginsenosides Rg1 and Rb1 on depression-and anxiety-like behaviors in chronic unpredictable stress-induced rats.Methods Seventy male SPF grade SD rats were tested for sugar and water preference after 5 days of adaptation and divided into seven groups according to their preference index:a control group,model group,fluoxetine hydrochloride group,ginsenoside Rg1 24 mg/kg group,ginsenoside Rg1 48 mg/kg group,ginsenoside Rb1 33 mg/kg group,and ginsenoside Rb1 67 mg/kg group.All rats,except for the control group,were subjected randomly to one or two different stimulating factors every day for a total of 35 days.On the 36th day,behavioral experiments including sugar and water preference,open field,novel environment feeding inhibition,elevated cross maze,and forced swimming experiments were conducted to investigate the anti-depression and anti-anxiety effects of the treatments.Serum and hippocampal levels of interleukin(IL)-1β,IL-6,tumor necrosis factor(TNF)-α and serum corticosterone were measured by enzyme-linked immunosorbent assay.Results Compared with the model group,ginsenoside Rg1 and Rb1 significantly increased sucrose consumption in the sucrose preference test and decreased immobility in the forced swimming test.Ginsenoside Rg1(48 mg/kg)significantly reduced the latency to eat in the novelty-suppressed feeding test,and ginsenoside Rg1(24 and 48 mg/kg)significantly increased the percentage of open arm entries and time in the elevated cross maze test.Serum corticosterone levels were significantly decreased in the ginsenoside Rg1 and Rb1 groups,serum IL-1β and IL-6 levels were significantly decreased in the ginsenoside Rg1(48 mg/kg)group,serum TNF-α and IL-6 levels were significantly decreased in the ginsenoside Rb1(33 mg/kg)group,and IL-1β,IL-6,and TNF-α levels in the hippocampus were significantly decreased in the ginsenoside Rg1(48 mg/kg)and Rb1(67 mg/kg)groups.Conclusions Both ginsenosides can regulate the hypothalamic-pituitary-adrenal axis and inhibit neuroinflammation,improving depression-and anxiety-like behaviors in rats induced by chronic unpredictable stress.Ginsenoside Rg1 has a significantly better anti-anxiety effect than Rb1.
