Progress in the optimization of CRISPR/Cas9 technology for the establishment of genetically modified mouse models
10.3969/j.issn.1671-7856.2024.05.017
- VernacularTitle:利用CRISPR/Cas9技术构建基因修饰小鼠模型的技术优化进展
- Author:
Yu WANG
1
;
Yong WU
;
Chunnan LIANG
;
Changfa FAN
Author Information
1. 中国食品药品检定研究院实验动物资源研究所,北京 102629
- Keywords:
CRISPR/Cas9;
mouse model;
conditional knockout/knockin;
delivery
- From:
Chinese Journal of Comparative Medicine
2024;34(5):152-158
- CountryChina
- Language:Chinese
-
Abstract:
CRISPR/Cas9 technology has driven the development of various fields in life science.With continuous deepening of its understanding,researchers have made multiple improvements and optimizations to adapt to various application scenarios.The optimization of CRISPR/Cas9 technology has also provided breakthroughs in the establishment of genetically modified mouse models.This article briefly reviews the development process of CRISPR/Cas9 technology and summarizes optimization strategies of CRISPR/Cas9,establishment of conditional knockout/knockin gene-modified mouse models,and delivery systems for CRISPR/Cas9 elements and HDR templates.
