LIMK1 promotes the progression of cervical cancer by up-regulating the ROS/Src pathway
10.3969/j.issn.1671-7856.2024.05.010
- VernacularTitle:LIMK1通过上调ROS/Src通路进而促进宫颈癌的进展
- Author:
Yajing JIA
1
;
Naiyi DU
;
Wei ZHAO
;
Yongping LI
;
Yakun LIU
Author Information
1. 河北医科大学第四医院妇科,石家庄 050011
- Keywords:
LIMK1;
ROS;
Src;
cervical cancer;
RUNX3
- From:
Chinese Journal of Comparative Medicine
2024;34(5):92-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of LIMK1 on the progression of cervical cancer(CC).Methods HeLa and C-33A human cervical cancer cells overexpressing LIMK1 were established and injected subcutaneously into nude mice.The tumor volume was measured and expression of NOX2,NOX4,p-Src,p-RUNX3,RUNX3,and MMP-9 proteins in tumor cells was detected by Western blot assays.LIMK1-overexpressing HeLa and C-33A cells were cultured in 5％O2 with antioxidants.The protein expression of LIMK1,NOX4,p-Src,p-RUNX3,RUNX3 and MMP-9 in the cells was detected by Western blot assays.Cell migration was assessed by a scratch assay.Transwell assays were used to assess cell migration and invasion.A monoclonal proliferation assay was used to assess cell proliferation.Results The tumor volume in nude mice injected with LIMK1-overexpressing HeLa cells was increased significantly,and NOX2,NOX4,p-Src,p-RUNX3 and MMP-9 protein levels were increased,while RUNX3 protein expression was decreased.In LIMK1-overexpressing HeLa and C-33A cells,the protein expression of LIMK1,NOX4,p-Src,p-RUNX3,and MMP-9 was increased,RUNX3 protein expression was decreased,and cell migration,invasion,and proliferation were increased.However,after adding antioxidants,the expression levels of NOX4,p-Src,p-RUNX3,RUNX3 and MMP-9 proteins,and cell migration,invasion,and proliferation were not different from those of control cells.Conclusions LIMK1 promotes the progression of cervical cancer by enhancing the ROS/Src pathway,thereby promoting the migration,invasion,and proliferation of cervical cancer cells.
