Effects of acute sleep deprivation on behavior and synaptic biomarker expression in rats
10.3969/j.issn.1671-7856.2024.05.006
- VernacularTitle:不同时间急性睡眠剥夺对大鼠行为学及突触生物标志物表达的影响
- Author:
Shibin ZHANG
1
;
Lu WANG
;
Chu WANG
;
Pengcheng GUO
;
Xusheng YAN
;
Dongsheng HUO
;
Zhanjun YANG
;
Yanguo WANG
;
Jianxin JIA
Author Information
1. 内蒙古科技大学包头医学院研究生学院,内蒙古包头 014040
- Keywords:
rat;
sleep deprivation;
learning cognition;
hippocampus;
synaptic plasticity
- From:
Chinese Journal of Comparative Medicine
2024;34(5):55-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of acute sleep deprivation on the behavior and synaptic protein expression of rats.Methods Seventy healthy male Wistar rats were randomly divided into seven groups,a Control group and sleep deprivation groups(24,48,72,96,120 and 144 hours).The sleep deprivation rat model was established by the modified multiplatform water environment sleep deprivation method.Spatial learning and memory were assessed by the Morris water maze.Anxiety was assessed by the open field test.The morphology and quantity of hippocampal neurons were observed by Nissl staining.Western blot and Real-time PCR were used to determine the expression of synaptophysin(SYN),post-synaptic density protein-95(PSD-95),and brain-derived neurotrophic factor(BDNF)in rats.Results Compared with the Control group,the numbers of standing and modification were significantly increased by prolongation of the sleep deprivation time(P＜0.05).The escape latency and path length were significantly increased in 120 and 144 h groups(P＜0.05),whereas the number of platform crossings and the percentage of the target quadrant time were significantly decreased(P＜0.01)and negatively correlated to the sleep deprivation time.The expression levels of BDNF,SYN,and PSD-95 were significantly decreased with the prolongation of sleep deprivation time(P＜0.01).Conclusions With the increase in sleep deprivation time,cognitive dysfunction and anxiety gradually deteriorated,which may be related to decreases in the expression of synaptic biomarkers.
