Comparison of liver and adrenal transplantation models of neuroblastoma

VernacularTitle:神经母细胞瘤肝及肾上腺移植模型比较
Author: Hongxia CHEN ^{1
,

2} ; Zhigang TAN ; Huiran LIN ; Luping FENG ; Chuya ZHENG ; Wenfeng LIAO ; Rufeng ZENG ; Jinxin LIU ; Zhenjian ZHUO
Author Information

1. 北京大学深圳研究生院实验动物中心,广东深圳 518055
2. 省部共建肿瘤化学基因组学国家重点实验室,广东深圳 518055
Keywords: neuroblastoma; orthotopic adrenal transplantation; liver transplantation; disease model; rate of tumor formation
From: Chinese Journal of Comparative Medicine 2024;34(4):100-108
CountryChina
Language:Chinese
Abstract: Objective A neuroblastoma(NB)liver transplantation model was established and compared with the adrenal orthotopic transplantation model to explore its characteristics.Methods 5× 105 SK-N-SH cells were implanted along the long axis of the left lobe of mouse livers with a micro-injection needle.The growth,metastasis,expression of related genes,and histopathological changes of tumors were detected after the modeling.Results The tumor formation rate in mice inoculated with tumor cells reached 100％after 21 days,and tumor growth,metastasis,related gene expression changes,and pathological characteristics were apparent.Conclusions In this study,a neuroblastoma liver transplantation model was successfully constructed via a relatively simple surgical method to provide a more suitable choice for future scientific NB experiments.