A comparative study of enzymatic digestion methods for the preparation of single-cell suspensions from the lamina propria of the mouse intestine
10.3969/j.issn.1671-7856.2024.04.011
- VernacularTitle:小鼠肠道固有层单细胞悬液制备的酶消化法比较研究
- Author:
Qin ZENG
1
;
Ying LIANG
;
Xinhui WANG
;
Lei YAN
;
Xiangpeng WANG
;
Jiayi YANG
;
Renhuan YU
Author Information
1. 中国中医科学院西苑医院肾病科,北京 100091
- Keywords:
intestinal lamina propria;
single-cell suspensions;
enzyme digestion;
flow cytometry assay
- From:
Chinese Journal of Comparative Medicine
2024;34(4):92-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the optimal digestion method for the preparation of single-cell suspensions from mouse small intestinal lamina propria.Methods Ten mouse small intestines of uniform length were collected and randomly divided into two groups.Each group was used to prepare lamina propria single-cell suspensions by enzymatic digestion with collagenase A or collagenase Ⅷ.We compared the effects of these two enzymatic digestion method on the cell yield,cell activity,and cell surface antigens of the single-cell suspensions.Single-cell suspensions prepared by the superior enzymatic digestion method were then subjected to flow cytometry assay.Results Compared to collagenase-Ⅷ-based enzymatic digestion,collagenase-A-based digestion result ed in a higher cell yield(9.48±1.10)× 109vs(4.18±1.02)×109and higher proportions of live cells(86.36±3.32)％vs(61.62±10.93)％,active CD45+cells(57.19±5.11)％vs(26.01±11.44)％,active CD3+cells(8.73±2.89)％vs(4.52±2.49)％,active CD4+cells(6.19±2.09)％vs(3.22±1.91)％,and active B220+cells(15.06±4.27)％vs(5.07±2.20)％,providing high-quality cells for subsequent flow assays.Conclusions The collagenase A-based enzymatic digestion method is more suitable for the preparation of ingle-cell suspensions from the lamina propria of ouse small intestines.
