Establishment of chimeric rabbits with FOXN1 gene knockout
10.3969/j.issn.1671-7856.2024.04.005
- VernacularTitle:FOXN1基因敲除兔嵌合体的建立
- Author:
Tianping LIU
1
,
2
;
Guiling LI
;
Ke LIU
;
Bangzhu CHEN
;
Gang WANG
;
Weiwang GU
Author Information
1. 五邑大学药学与食品工程学院,广东省医学大动物模型重点实验室,广东江门 529020
2. 广东省医学实验动物中心,南方医科大学附属广东省人民医院(广东省医学科学院),广东佛山 528248
- Keywords:
FOXN1;
gene knockout rabbit;
immune deficiency;
chimera
- From:
Chinese Journal of Comparative Medicine
2024;34(4):35-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish F0 generation chimeric rabbits with FOXN1 gene knockout and explore method for the in vivo conservation of immunodeficient rabbits in a conventional housing environment.Methods Initially,CRISPR/Cas9 technology was employed to inject constructed sgRNA and Cas9 protein into a single cell from rabbit two-cell stage embryos to obtain chimeric embryos with FOXN1 gene editing.The embryos were subsequently transferred into surrogate does.Finally,the F0 generation offsprings were genotyped using PCR and Sanger sequencing,and their growth and development in a conventional housing environment were observed.Results The PCR and Sanger sequencing result confirmed the successful establishment of himeric rabbits with FOXN1 gene knockout.On observation,the chimeras exhibited normal growth and development in a conventional environment without any immunodeficient phenotypes.Conclusions This study established a preliminary chimeric rabbit model with FOXN1 gene knockout that grows and develops normally in standard laboratory environments.This lays the foundation for the further breeding of FOXN1 immunodeficient rabbits in the future.
