Analysis of immune cells and their subsets in peripheral blood and spleen of CAST/EiJ mice
10.3969/j.issn.1671-7856.2024.04.001
- VernacularTitle:CAST/EiJ小鼠外周血和脾中免疫细胞及亚群分析
- Author:
Qi LIU
1
;
Yongzhi HOU
;
Na LI
;
Jingjing ZHANG
;
Jiahan LU
;
Zhe CONG
;
Lin ZHU
;
Jing XUE
Author Information
1. 中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,国家卫生健康委员会人类疾病比较医学重点实验室,新发再发传染病动物模型研究北京市重点实验室,北京 100021
- Keywords:
CAST/EiJ mice;
C57BL/6J mice;
classical dendritic cells;
natural killer cells;
B lymphocytes;
T lymphocytes
- From:
Chinese Journal of Comparative Medicine
2024;34(4):1-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possible causes of CAST/EiJ mouse susceptibility to multiple pathogens,the immune cell phenotypes in the peripheral blood and spleen of CAST/EiJ mice were analyzed to clarify their composition.Methods Classical dendritic cells(cDCs),natural killer(NK)cells,B lymphocytes,T lymphocytes,and their subsets in the peripheral blood and spleen of CAST/EiJ mice and C57BL/6J mice were detected by flow cytometry using the cell surface markers CD3,CD4,CD8,CD11b,CD11c,CD19,CD27,CD49b,and TCRβ.Results There was no significant difference in the proportion of cDCs between CAST/EiJ and C57BL/6J mice,but the cDC1 cell subset population was smaller in CAST/EiJ.The proportions of NK cells(mainly mature NK cell subsets)and T lymphocytes(mainly CD8+T cells)were both lower in CAST/EiJ mice than C57BL/6J mice,while the proportion of B cells was higher in CAST/EiJ mice than C57BL/6J mice.Conclusions The proportions of NK and T lymphocytes in CAST/EiJ mice were lower than those in C57BL/6J mice.
