Study on a mouse model of aldosterone-induced multi-organ damage
10.3969/j.issn.1005-4847.2024.08.011
- VernacularTitle:醛固酮诱导多器官损害小鼠模型的研究
- Author:
Yu LUO
1
;
Haitao ZHANG
;
Yawei ZHENG
;
Xianze MENG
;
Zhen FANG
;
Yating WANG
;
Zhuyuan FANG
Author Information
1. 南京中医药大学附属医院,南京 210029
- Keywords:
aldosterone;
multiple organ damage;
injury of the kidney;
injury of the heart;
injury of the liver;
mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(8):1045-1051
- CountryChina
- Language:Chinese
-
Abstract:
Objective Establishment and evaluation of a mouse model of aldosterone-induced multi-organ damage.Methods Twenty mice were randomly divided into four groups,with five mice in each group:a blank control group(0 μg/(kg·d)),a low-dose aldosterone group(150 μg/(kg·d))),a medium-dose aldosterone group(300 μg/(kg d)),and a high-dose aldosterone group(450 pug/(kg·d)).Aldosterone-containing osmotic minipumps were surgically implanted under the skin,and aldosterone was infused for 4 weeks to establish the aldosterone-induced damage model.The body weight and blood pressure of the mice were recorded weekly.After the 4 week modeling period,the mice were euthanized,and their tissues were collected for observation and analysis of blood pressure and histological morphology of various organs.Results(1)After 4 weeks of aldosterone infusion,the serum aldosterone levels were significantly increased in the medium-dose and high-dose aldosterone groups,but not in the low-dose aldosterone group.(2)After the implantation of osmotic minipumps,the systolic blood pressure was significantly increased in the low-dose,medium-dose,and high-dose aldosterone groups during the second and third weeks,but decreased in all these groups during the fourth week.(3)The kidney and heart in the low-dose,medium-dose,and high-dose aldosterone groups showed varying degrees of damage,interstitial edema,collagen deposition,and fibrotic lesions.The liver in the low-dose aldosterone group showed a small amount of collagen deposition,while the medium-dose and high-dose aldosterone groups showed varying degrees of hepatocyte damage,collagen deposition,and fibrotic lesions.Conclusions Aldosterone can induce multi-organ damage in mice.Under this modeling method,organ damage is mainly manifested as edema,collagen deposition,and fibrotic lesions.
