Evaluation of inhalation induction of anesthesia for tracheal intubation in miniature pigs
10.3969/j.issn.1005-4847.2024.08.010
- VernacularTitle:小型猪气管插管时异氟醚半数有效剂量的探索
- Author:
Yan LENG
1
;
Na DAI
;
Qianqian QIAO
;
Xiaoshuai ZHAO
;
Hao TIAN
;
Mingxia FAN
Author Information
1. 武汉大学人民医院麻醉科,武汉 430062
- Keywords:
large animal experiment;
miniature pigs;
anesthesia;
isoflurane;
endotracheal intubation;
minimum alveolar concentration
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(8):1039-1044
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study was performed to explore the use of intramuscular low-dose Zoletil(1.5 mg/kg)combined with isoflurane inhalation for tracheal intubation in miniature pigs while preserving spontaneous respiration by determining the 50％and 95％minimum alveolar concentrations effective inhaled(MAC EI50 and MAC EI95).The goal was to establish a safe anesthetic method for tracheal intubation in miniature pigs in which intubation is difficult.Methods Forty-four Bama miniature pigs underwent general anesthesia.Following sedation with an intramuscular injection of sufentanil,anesthetic induction was performed using mask inhalation of isoflurane with monitoring of the heart rate,blood pressure,respiration,body temperature,oxygen saturation,end-tidal carbon dioxide concentration,and end-tidal isoflurane concentration.The initial end-tidal isoflurane concentration was set at 2.0％.The tracheal intubation conditions and outcomes were evaluated using Cooper's scoring system,and the dose-response relationship was calculated using Dixon's up-and-down method.Probit regression was employed to calculate the MAC EI50 and MAC EI95 along with their 95％confidence intervals(CI).Results In tracheal intubation using direct laryngoscopy with induction by low-dose sufentanil combined with isoflurane inhalation in miniature pigs,the MAC EI50 was 3.10％(95％CI,2.79％～3.56％)and the MAC EI95 was 3.77％(95％CI,3.41％～6.42％).With proper monitoring and airway management planning in place,alveolar isoflurane concentrations ranging from 3.10％to 3.75％were able to maintain stable vital signs in the miniature pigs.Conclusions The use of Zoletil combined with isoflurane inhalation for tracheal intubation in miniature pigs,aimed at preserving spontaneous breathing,is a preferable and safe anesthetic method for oral airway management in miniature pigs with significant potential for widespread application.
