Effect of acute ozone exposure on miRNA expression profile of exosomes in alveolar lavage fluid of rats
10.3969/j.issn.1005-4847.2024.07.003
- VernacularTitle:急性臭氧暴露对大鼠肺泡灌洗液中外泌体miRNA表达谱的影响
- Author:
Qingcheng YANG
1
,
2
;
Jiao ZHAO
;
Renxin YANG
;
Zhangong YAN
;
Zhuge XI
;
Xiaohua LIU
Author Information
1. 天津体育学院运动健康学院天津市运动生理学与运动医学重点实验室,天津 301617
2. 军事科学院军事医学研究院环境医学与作业医学研究所,天津 300050
- Keywords:
ozone;
acute exposure;
exosomes;
miRNA;
bronchoalveolar lavage fluid
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(7):836-845
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the changes in the microRNA(miRNA)expression profile of rat alveolar lavage-fluid-derived exosomes after acute ozone exposure and analyze its potential relationship with lung injury.Methods Adult male Wistar rats were exposed to 0 ppm,0.5 ppm,and 2 ppm ozone for 6 h in a gas exposure chamber.After 24 h,the rats were anesthetized,the alveolar lavage fluid was collected,and exosomes were extracted.Differentially expressed miRNAs in exosomes were detected by miRNA-seq technology.miRDB databases were used to predict the target genes of the top 10 differentially expressed miRNAs among the groups,and Gene Ontology function enrichment and KEGG Pathway analyses were performed on the target genes.The differentially expressed miRNAs with high expression abundance and good uniformity were verified by quantitative reverse-transcription PCR(qRT-PCR).Results Compared with the findings in the 0 ppm group,there were 8 up-regulated and 55 down-regulated miRNAs in the 0.5 ppm group,and 32 up-regulated and 61 down-regulated miRNAs in the 2 ppm group.Compared with the findings in the 0.5 ppm group,90 exosomal miRNAs were up-regulated and 74 were down-regulated in the 2 ppm group.The target genes of top 10 differentially expressed miRNAs were mainly involved in regulating DNA binding,heterocyclic compound binding,and affecting transcription,and were closely related to cancer,MAPK,PIK3-AKT,TNF,ErbB,mitophagy,and FoxO signaling pathways.The qRT-PCR showed that the sequencing result were basically reliable.Conclusions Acute ozone exposure can lead to changes in the expression profile of exosomal miRNA in the alveolar lavage fluid of rats.Differentially expressed miRNAs may be involved in pathological processes,such as lung tissue inflammation and lung injury,caused by ozone exposure.
