Ifitm3 knockout inhibits the proliferation and differentiation of neural stem cells in mice
10.3969/j.issn.1005-4847.2024.06.002
- VernacularTitle:Ifitm3敲除抑制小鼠神经干细胞增殖和分化
- Author:
Kaiyu WANG
1
;
Xuepei LEI
;
Yiying HUANG
;
Guiying SHI
;
Hanwei YUE
;
Jie WANG
;
Yifan LIN
;
Jiaming TANG
;
Lin BAI
Author Information
1. 中国医学科学院医学实验动物研究所,国家卫生健康委员会人类疾病比较医学重点实验室,国家人类疾病动物模型资源库,北京 100021
- Keywords:
interferon-induced transmembrane protein 3;
gene knockout;
neural stem cells;
proliferation;
neural differentiation
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(6):691-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish interferon-induced transmembrane protein 3(Ifitm3)knockout mice and to explore the effects of Ifitm3 on the proliferation and differentiation of adult neural stem cells of mice(aNSCs).Methods IFITM3 knockout mice were established by the CRISPR/Cas9 method and identified by genotype identification and Western Blot.The differences between Ifitm3-knockout mice and wild-type mice were analyzed by hematoxylin-eosin(HE)staining and flow cytometry.The aNSCs of wild-type mice and Ifitm3-knockout mice were isolated and cultured,the number and size of neurospheres were detected,The ability of aNSCs to proliferate and differentiate were detected by quantitative reverse-transcription polymerase chain reaction,Western Blot,and immunofluorescence.Results Ifitm3-knockout mice were successfully established.The mice developed normally,and there were no obvious abnormalities either histopathologically or the immune system.In vitro experiments showed that Ifitm3 knockout inhibited the self-renewal potential of aNSCs,led to a decrease in the proliferation ability of aNSCs,and inhibited the differentiation of aNSCs into immature neurons and astrocytes.Conclusions This study finds that a lack of IFITM3 result in the ability of aNSCs to proliferate and differentiate decreased,IFITM3 may regulate the function of aNSCs.
