Establishment of a genetically diverse mouse model of hypertension and analysis of gene transcription regulation
10.3969/j.issn.1005-4847.2024.05.004
- VernacularTitle:遗传多样性高血压小鼠模型的建立与基因转录调控分析
- Author:
Zhibin HUANG
1
;
Jirong PAN
;
Lingyan ZHANG
;
Dalu ZHAO
;
Qian WANG
;
Chengzhi WEI
;
Xu MA
;
Lin BAI
;
Chuan QIN
Author Information
1. 中国医学科学院北京协和医学院医学实验动物研究所,北京 100021
- Keywords:
genetically diverse mouse;
Cckbr;
essential hypertension;
animal model
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(5):576-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differences in blood pressure phenotypes,renal pathological changes,and related pathogenic pathways in genetically diverse hypertensive mice obtained from 13 strains.Methods The genotypes of Cckbr+/+,Cckbr+/-and Cckbr-/-were obtained by hybridization of 13 strains of genetically diverse mice with Cckbr-/-mice.Blood pressure was measured with a noninvasive blood pressure analysis system(BP-2000).The expression of CCKBR protein in mouse kidney tissue was detected by Western Blot,and the pathological changes in mouse kidney tissue were detected by hematoxylin-eosin(HE)staining and immunohistochemistry(IHC).The pathogenic pathways related to essential hypertension were screened by RNA sequencing.Results In three specific mouse strains(A/J,LOT,and FIM),the systolic blood pressure(SBP)was significantly different between the Cckbr-/-and Cckbr+/+groups.HE staining and IHC showed that hypertension caused a certain degree of renal injury in the mice.Gene Ontology(GO)and pathway enrichment analysis showed that differentially expressed genes were enriched in metabolic processes and circadian rhythm regulation.Conclusions Genetically diverse mice can effectively simulate the genetic background of the population and provide a new resource for studying the pathogenic genes related to essential hypertension.
