Development of microsatellite markers and analysis of genetic characteristics of laboratory Apodemus peninsulae population

VernacularTitle:实验用大林姬鼠种群微卫星标记开发及遗传特性分析
Author: Qing ZHANG ¹ ; Xiwen ZHANG ; Song HE ; Bao YUAN ; Jian CHEN ; Wenzhi REN ; Fushi QUAN ; Jinping HU ; Yu DING
Author Information

1. 吉林大学动物科学学院,长春 130062
Keywords: Apodemus peninsulae; microsatellite loci; genetic diversity; closed colony
From: Acta Laboratorium Animalis Scientia Sinica 2024;32(4):468-476
CountryChina
Language:Chinese
Abstract: Objective Polymorphic microsatellite markers developed for Apodemus peninsulae can enrich its genetic data and lay a foundation for genetic quality control and gene mapping.Methods Microsatellite loci were screened based on the genome sequence of Apodemus peninsulae,and microsatellite primers were identified.The genetic diversity of the population was analyzed by multiplex PCR.Results Thirty microsatellite markers were successfully developed and evaluated using 60 samples of Apodemus peninsulae.A total of 152 alleles were detected,with an average of 5.067 alleles per locus.The average observed heterozygosity was 0.592.The average Shannon index was 1.265.The average polymorphism information content was 0.598.Conclusions Based on the microsatellite loci developed in this study,the genetic diversity of Apodemus peninsulae can be effectively analyzed,laying a foundation for establishing genetic quality standards and detection method.