Establishment of a lipotoxic inhibition model of bone formation in zebrafish induced by palmitic acid
10.3969/j.issn.1005-4847.2024.04.006
- VernacularTitle:棕榈酸诱导斑马鱼脂毒性损伤骨形成抑制模型的建立
- Author:
Xiaoyi WANG
1
,
2
;
Miao LI
;
Linxia WANG
;
Bin YU
;
Yongqing HUA
Author Information
1. 江苏省中药资源产业化过程协同创新中心,南京 210023
2. 南京中医药大学药学院,南京 210023
- Keywords:
osteoporosis model;
lipotoxicity;
zebrafish;
palmitic acid;
simvastatin
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(4):461-467
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a palmitic acid(PA)-induced model of lipotoxic bone formation inhibition in zebrafish.Methods AB strain zebrafish embryos were divided randomly into a blank control group,PA group,and simvastatin(SIM)group.Embryos in the PA and SIM groups received PA from 3 days post-fertilization(dpf),and embryos in the SIM group received SIM continuously for 4 days from 5 dpf.Establishment of the model was confirmed at 9 dpf by calcein staining,Nile red staining,triglyceride and total cholesterol content determination,and q-PCR.Results PA significantly decreased the number of vertebrae,promoted lipid accumulation,increased triglyceride and total cholesterol contents,promoted the expression of lipid-related genes PPARγ、c/EBPα,and inhibited the expression of osteogenic genes ALP and RUNX2.SIM improved the inhibitory effect of PA on bone formation in zebrafish.Conclusions PA can successfully create a lipotoxic model of bone-formation inhibition,similar to the pathological process of osteoporosis,using a simple,sensitive,and controllable method.This model can then be used for drug screening for osteoporosis and related diseases.
