Anti-inflammatory and analgesic effects of small intestine Ski-overexpression on acetic acid-induced writhing mice
10.3969/j.issn.1005-4847.2024.03.011
- VernacularTitle:小肠过表达Ski蛋白对扭体小鼠的抑炎镇痛作用
- Author:
Ao XIONG
1
,
2
;
Xiaoyu WEI
;
Yan PENG
;
Ming YANG
;
Qiandong YANG
;
Fei LUO
Author Information
1. 陆军军医大学(第三军医大学)第一附属医院骨科,重庆 400038
2. 陆军军医大学陆军特色医学中心野战外科研究部,重庆 400042
- Keywords:
Ski;
writhing test;
IL-1β;
TNF-α;
nerve growth factor;
tyrosine protein kinase A;
mice
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(3):369-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-inflammatory and analgesic effects of Ski protein overexpression on writing in mice induced by acetic acid.Methods Eight-week-old male ICR mice were administered 0.7%acetic acid solution(0.1 mL/10 g)to induce a writhing reaction.The mice were divided into sham,acetic acid,acetic acid+ibuprofen,acetic acid+ad-EGFP,acetic acid+ad-ski-1,acetic acid+ad-ski-2,and acetic acid+sulfasalazine groups(n=10 mice per group).The time to the first appearance of twisting and the number of twists within 15 min were recorded.Small intestine tissues were removed to identify the effect of adenovirus transfection and to detect protein expression levels of pro-inflammatory factors and pain biomarkers and protein expression of nuclear factor(NF)-κB p65 and its binding with Ski protein.Results Ski protein was successfully overexpressed in small intestine after intraperitoneal injection of Ad-ski adenovirus.Overexpressed Ski protein delayed the start and decreased the frequency of writhing,comparable to ibuprofen(P＞0.05).Groups in which ski protein was overexpressed showed significantly inhibited protein expression of pro-inflammatory factors and pain biomarkers compared with the acetic acid group(P＜0.05).Moreover,NF-κB p65 formed complexes with Ski.Conclusions Overexpression of Ski protein has anti-inflammatory and analgesic effects on acetic acid-induced inflammatory pain by inhibiting the expression of inflammatory factors and pain biomarkers,via regulation of the NF-κB signaling pathway.
