From Phenotypes to Molecules:Revolutionizing Gut Microbiota Identification Methods
10.13865/j.cnki.cjbmb.2024.03.1470
- VernacularTitle:从表型到分子:革命性的肠道菌群鉴定方法
- Author:
Xuan WANG
1
,
2
,
3
;
Chang-Long LV
;
Jing-Bo ZHAI
Author Information
1. 内蒙古民族大学医学院,内蒙古通辽 028000
2. 延边大学化学生物学研究中心,吉林延吉 133002
3. 人兽共患病防控自治区高等学校重点实验室,内蒙古 通辽 028000
- Keywords:
gut microbiota;
16S rRNA;
real-time fluorescent qPCR;
high-throughput sequencing;
mass spectrum
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(8):1065-1077
- CountryChina
- Language:Chinese
-
Abstract:
The gut microbiota is a complex ecosystem composed of many bacteria and their metabolites.It plays an irreplaceable role in human digestion,nutrient absorption,energy supply,fat metabolism,im-mune regulation,and many other aspects.Exploring the structure and function of the gut microbiota,as well as their key genes and metabolites,will enable the early diagnosis and auxiliary diagnosis of disea-ses,new treatment methods,better effects of drug treatments,and better guidance in the use of antibiot-ics.The identification of gut microbiota plays an important role in clinical diagnosis and treatment,as well as in drug research and development.Therefore,it is necessary to conduct a comprehensive review of this rapidly evolving topic.Traditional identification methods cannot comprehensively capture the di-versity of gut microbiota.Currently,with the rapid development of molecular biology,the classification and identification methods for gut microbiota have evolved from the initial phenotypic and chemical identi-fication to identification at the molecular level.This review integrates the main methods of gut microbiota identification and evaluates their application.We pay special attention to the research progress on molec-ular biological methods and focus on the application of high-throughput sequencing technology in the iden-tification of gut microbiota.This revolutionary method for intestinal flora identification heralds a new chapter in our understanding of the microbial world.
