Development and Application of RNA-cleaving Deoxyribozyme Catalytic Amplification in MicroRNA Biosensors
10.13865/j.cnki.cjbmb.2024.01.1419
- VernacularTitle:脱氧核酶催化放大效应在微RNA传感中的研究与应用
- Author:
Shu-Ji WANG
1
;
Ru-Fei CAO
;
Xiao-Lei DUAN
Author Information
1. 遵义医科大学附属医院医学检验科,贵州遵义 563000
- Keywords:
microRNA(miRNA);
RNA-cleaving deoxyribozyme(RCD);
DNA nano-self-assembly;
isothermal amplification;
nanomaterials
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(7):889-896
- CountryChina
- Language:Chinese
-
Abstract:
MicroRNAs(miRNAs)are small non-coding RNAs that are closely associated with the occur-rence and progression of tumors and other diseases.However,miRNAs require high-sensitivity and high-specificity detection due to their low abundance,high sequence homology,and rapid degradation.The RNA-cleaving deoxyribozyme(RCD)is a functional single-stranded DNA molecule that enables specific cleavage of substrates to release miRNAs that can be recycled with the assistance of metal ions,prompting cyclic signal amplification.Recently,developing new methods based on RCD catalytic amplification for miRNA high-sensitivity detection has become the focus of researchers.Based on combing with the various new technologies and materials in miRNA biosensors,this study classifies and reviews the new methods for detecting miRNAs based on deoxyribozyme catalytic amplification developed in recent years.We sepa-rate these miRNA detection strategies into three categories:RCDs combined with DNA self-assembly,i-sothermal amplification,and nanomaterials.We explore the basic principles of each approach,the latest research advancements,and application scenarios in biomedical sensors and medical detection.This re-view provides a foundation and reference for further research of highly sensitive and accurate miRNA de-tection strategies.
