Changes of 5-hydroxytryptamine and tryptophan hydroxylase expression in the ventral horn of spinal cord
- VernacularTitle:运动性疲劳后脊髓前角5-羟色胺和色胺酸羟化酶表达的变化
- Author:
XU CHUAN-XIANG
1
;
LIU HONG-TAO
;
WANG JING
Author Information
1. 军事医学科学院卫生学环境医学研究所
- Keywords:
ventral horn;
spinal cord;
5-hydroxytryptamine;
tryptophan hydroxylase;
exercise-induced fatigue;
immunohistochemistry
- From:
Neuroscience Bulletin
2008;24(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate changes of 5-hydroxytryptamine (5-HT) and its synthesis rate-limiting enzyme tryptophan hydroxylase (TPH) in the ventral horn of spinal cord after exercise-induced fatigue, and to further discuss the mechanism of exercise-induced central fatigue at spinal level. Methods Sixteen healthy adult Wistar rats were randomly divided into 2 groups: exercise-induced fatigue group and control group. Immunohistochemical staining for 5-HT and TPH in the ventral horn were performed and analysized quantitatively. The mean optic densities of 5-HT and TPH positive fibers or terminals were measured by computerized image analyzer. Results Both 5-HT and TPH positive fibers/terminals decreased in the exercise-induced fatigue group. The immunohistochemical staining was weaker and the mean optic densities decreased obviously in the fatigue group compared with those in the control group (P ＜ 0.05). Conclusion 5-HT and TPH in the ventral horn of spinal cord might be involved in exercise-induced fatigue.
