Insulin aggravates amyloid deposition and cognition impairment in an AD-like rat model
- VernacularTitle:胰岛素干预加重海马注射Aβ阿尔茨海默病大鼠模型认知损害
- Author:
Zhao QIAN-HUA
1
;
Luo YU-MIN
;
Zhou BIN
;
Hong ZHEN
Author Information
1. 复旦大学
- Keywords:
Alzheimer disease;
β-amyloid peptide;
insulin;
insulin-degrading enzyme;
animal model;
rat
- From:
Neuroscience Bulletin
2005;21(6):408-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective to evaluate the impact of insulin on pathology and cognition in AD-like rat model. Methods 32 male Wistar rats were divided into 4 groups: Aβ, Aβ+ insulin, insulin and normal control group. Each group included 8 subjects. Aggregated Aβ solution was injected in CA3 area of bilateral hippocampi. Insulin was administered subcutaneously pre-surgery once a day for 7 consecutive days. Behavioral performance in Morris water maze was observed and level of Aβ deposition was assessed by immunohistochemistry staining and ELISA assay. Results Aβ+insulin group had a much higher Aβ level (increased by 23.6%) and more severe Aβ deposition (increased by 35.7%) than the other three groups. And poorer performance in Morris water maze was observed. Conclusion Insulin can aggravate Aβ deposition in the hippocampus of AD-like rat model and worsen its memory loss.
