Expression of ADM and ADM mRNA in the cerebral cortex after focal cerebral ischemia/reperfusion in rats
- VernacularTitle:大鼠局灶性缺血再灌注后大脑皮质ADM及其mRNA的表达
- Author:
Bi GUO-RONG
1
;
Zhang HUI
;
Zhou HUI-JIE
;
Zhang HE-MIN
;
Hai HONG
;
Fang XIU-BIN
Author Information
1. 中国医科大学附属第二医院(盛京医院)
- Keywords:
cerebral ischemia/reperfusion;
adrenomedullin;
immunohistochemical method;
RT-PCR method
- From:
Neuroscience Bulletin
2005;21(6):391-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the relationship between ADM and the injury of focal cerebral ischemia/reperfusion. Methods Construct the model of the rat middle cerebral artery ischemia/reperfusion by the line-block-method which the reperfusion is applied after the ischemia lasting for 2 h. By the method of immunohistochemistry and RT-PCR, we test the expression of ADM and ADM mRNA after focal cerebral ischemia/reperfusion in the brain of rats in different time, and make a dynamic observation at the same time. Results ADM and ADM mRNA express in the normal rat's brain, but lower than the sham operated group, P>0.05. There is a over expression of ADM and ADM mRNA after focal cerebral ischemia/reperfusion in the brain of rats, the difference is obviously compared with the normal group and the sham operated group, P<0.05. After the ischemia last for 2 h, the dynamic observation showed that ADM and ADM mRNA began to express in the cerebral cortex when the reperfusion last for 2 h after, and reach the peak time at 22 h, and still more than the normal group at the time of 1 w, P<0.05. Conclusion ADM and ADM mRNA showed a regularitily over expression after ischemia/reperfusion of the brain.
