Effect of a antisense oligonucleotide to noggin on the expression of nestin and GFAP in the hippocampus of adult rats
- VernacularTitle:反义Noggin基因对成年大鼠海马内Nestin及GFAP表达的影响
- Author:
Xu HAI-WEI
1
;
Fan XIAO-TANG
Author Information
1. The Third Military Medical University
- Keywords:
immunohistochemistry;
rat;
noggin;
hippocampus;
nestin;
GFAP
- From:
Neuroscience Bulletin
2005;21(5):319-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the role of noggin on the expression of nestin and glial fibrillary acidic protein (GFAP) in the hippocampus of adult rats. Methods Antisense oligodeoxynucleotide (ASODN) technique was employed to inhibit endogenous noggin expression and immunohistochemistry was used to detect the expressions of Nestin and GFAP in the hippocampus of adult rats. Results It was observed that the number of nestin and GFAP immunoreactive cells in the dentate gyrus (DG) of hippocampus was increased in adult rats treated with antisense oligodeoxynucleotide to noggin. Moreover, the number of GFAP immunoreactive cells was increased in the subventricular zone of the rats treated with antisense oligodeoxynucleotide to noggin. Conclusion The results in the present study indicates that noggin may play a role in the differentiation of neural stem cells in the adult hippocampus, and it promotes the differentiation of neural stem cells in the DG to neuronal fate.
