Expression of MAPK in the hippocampus of Alzheimer's disease rats model
- VernacularTitle:阿尔茨海默病大鼠模型海马 MAPK的表达
- Author:
Shang XIU-LI
1
;
Xu WAN-PENG
;
Lin YUN-HUI
;
Yang JIN-XIA
;
Zhang HUA
;
Xue YI-XUE
Author Information
1. 中国医科大学附属第一医院
- Keywords:
Alzheimer's disease;
amyloid-beta peptide;
long term potentiation;
mitogen activated protein kinase;
rat
- From:
Neuroscience Bulletin
2005;21(4):296-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of mitogen activated protein kinase (MAPK) in Alzheimer' s disease (AD) rat model. Methods AD rat model was established by injection of amyloid-beta peptide 1-40, 1 μl (10 μg/μl) into the hippocampus. Immunohistochemistry was used to detect the expression of MAPK protein. Learning and memory ability and LTP were determined by Morris water maze and electrophysiological methods respectively. Results The escape latency was increased in Alzheimer rats two weeks after injection of Aβ, compared with the control rats ( P ＜0.05); The slope alteration of field-excitory postsynaptic potentiation (fEPSP) between pre- and post- high-frequency stimulation(HFS) was decreased in Alzheimer rats (P ＜ 0.01 ). The augmented amplitude of fEPSP induced by HFS was lower in Alzheimer rats than in controls. The MAPK immunoreactive neurons in CA1 and CA3 areas of the hippocampus were less in number in Alzheimer rats than in control rats. Conclusion Aβ decreases the LTP and learning and memory ability by inhibiting MAPK signal pathway. MAPK may be involved in the pathogenesis of Alzheimer' s disease.
