Expression alterations of hypoxia-inducible factor-1 α subunit in the hippocampus of mice acutely and repeatedly exposed to hypoxia
- VernacularTitle:急性重复缺氧暴露小鼠海马 HIF-1α表达的变化
- Author:
Shao GUO
1
;
Zhang RAN
;
Gao CUI-YING
;
Lu GUO-WEI
Author Information
1. 首都医科大学
- Keywords:
hypoxic preconditioning;
hippocampus;
HIF-1;
mouse
- From:
Neuroscience Bulletin
2005;21(4):278-282
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effect of hypoxia preconditioning on HIF-1α expression in the hippocampus of mice during acute and repeated hypoxia exposures. Methods Mice were randomly divided into 3 groups and were exposed to hypoxia for 4 runs ( H4 group) , 1 run ( H1 group) and 0 run ( control group). RT-PCR, Western blot and EMSA were used to examine the HIF-1α expression in the mouse hippocampus following exposure to hypoxia. Total mRNA,total protein and nuclear protein were extracted from the mouse hippocampus for RT-PCR, Western blot and EMSA respectively. Results HIF-1α mRNA levels were found to be increased in H1 and decreased in H4 (vs H1 ). HIF-1 α protein levels and HIF-1 DNA binding activities were increased in H1 and markedly increased in H4. Conclusion HIF-1 activation may be involved in the protection of the brain of hypoxia-preconditioned mice.
