Expression of Nogo-66 receptor in primary cultured astrocytes
- VernacularTitle:Nogo-66受体分子在体外培养的星形胶质细胞中的表达
- Author:
Sun FANG
1
;
Jin WEI-LIN
;
Long MEI
;
Ju GONG
Author Information
1. 第四军医大学
- Keywords:
Nogo-66 receptor;
astrocyte;
in vitro;
expression
- From:
Neuroscience Bulletin
2005;21(4):273-277
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Nogo-66 receptor (NgR) in primary cultured rat astrocytes.Methods RT-PCR and Western blot were applied to verify mRNA and protein expression of NgR in primary cultured and purified astrocytes. Indirect immunofluorensence and confocal microscopic technique were used to study the distribution of NgR. Results Specific NgR cDNA product could be amplified from the total RNA of primary cultured astrocytes by RTPCR; Western blot of the extracts of astrocytes demonstrated a specific NgR band at about 64 kD. Indirect immunofluorescence and confocal scanning further revealed the intracellular localization of NgR protein in astrocytes. Simultaneously,the NgR protein was detected in C6 rat glioma cells by western blot and immunofluoresence staining. Conclusion NgR is expressed in primary cultured astrocytes, which offers strong and direct support for the expression of NgR in astrocytes in vivo.
